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Biochemistry

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JoVE Journal Biochemistry
Leaf Spray Mass Spectrometry
 

Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues

Article DOI: 10.3791/57949-v • 06:43 min • June 21st, 2018
June 21st, 2018 •
1, 2, 3, 1, 2
1Department of Horticultural Science, Microbial and Plant Genomics Institute, University of Minnesota, 2Department of Horticultural Science, Department of Plant and Microbial Biology, Microbial and Plant Genomics Institute, University of Minnesota, 3Department of Botany and Zoology, Stellenbosch University

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Leaf spray mass spectrometry is a direct chemical analysis technique that minimizes the sample preparation and eliminates chromatography, allowing for the rapid detection of small molecules from plant tissues.

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Leaf Spray Mass Spectrometry Ambient Ionization Technique Metabolites Plant Tissues Qualitative Analysis Semi-quantitative Analysis Sample Preparation Mesembrine Alkaloids Sceletium Plant Species Exogenous Chemicals Pesticides Protocol Optimization Nano Spray Source Fluidic Components Leaf Spray Mass Spectrometry Wire Voltage Application Clamp Soldering Sweep Cone Tune File Method File
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