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JoVE Journal
Biochemistry

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Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans
 

Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

Article DOI: 10.3791/57807-v 09:35 min October 15th, 2018
October 15th, 2018

Capitoli

Riepilogo

We describe a modified small-scale extraction and colorimetric assays of lactate and pyruvate in the nematode C. elegans. When utilizing commercial assay kits, the technical development of their sensitivity and accuracy is important. Protein precipitation in extraction is the most critical step for the quantitative determination of intracellular metabolites.

Tags

Keywords: Colorimetric Assay Lactate Pyruvate Caenorhabditis Elegans Nematode Protein Precipitation Homogenization E. Coli OP50 NGM Agar Plates Gravid Hermaphrodites Egg Isolation L1 Larvae Synchronous Culture Colorimetric Analysis Biochemical Methodology
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