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Biochemistry

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JoVE Journal Biochemistry
Contrast-Matching wasmiddel in Small-Angle Neutron Scattering experimenten voor membraan eiwit structurele analyse en modellering van de Ab Initio
 
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Contrast-Matching wasmiddel in Small-Angle Neutron Scattering experimenten voor membraan eiwit structurele analyse en modellering van de Ab Initio

Article DOI: 10.3791/57901-v • 10:27 min • October 21st, 2018
October 21st, 2018 •
1, 2, 1, 1, 2, 1
1Neutron Scattering Division, Oak Ridge National Laboratory, 2School of Chemistry and Biochemistry, Georgia Institute of Technology

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Dit protocol laat zien hoe een model met een lage resolutie ab initio en structurele details van een wasmiddel-ontbindend membraan eiwit in small-angle neutron scattering met contrast-matching van het detergent oplossing te verkrijgen.

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Biochemie kwestie 140 kleine hoek verstrooiing neutronen verstrooiing Biofysica membraan eiwit contrast variatie oppervlakteactieve stof micel intramembrane aspartyl protease presenilin signaal peptide peptidase
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