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秀丽线虫绿色荧光蛋白标记和 4 '、6-Diamidino-2-苯基吲哚染色检测蛋白质亚细胞定位
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Biologia
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JoVE Journal Biologia
Detecting Protein Subcellular Localization by Green Fluorescence Protein Tagging and 4′,6-Diamidino-2-phenylindole Staining in Caenorhabditis elegans
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秀丽线虫绿色荧光蛋白标记和 4 '、6-Diamidino-2-苯基吲哚染色检测蛋白质亚细胞定位

DOI:
10.3791/57914-v

•

09:36 min

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July 30, 2018

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, ,
1Department of Science,Borough of Manhattan Community College/CUNY

Capitoli

  • 00:04Titolo
  • 00:26Heat Stress
  • 02:46Ethanol Fixation and DAPI Staining
  • 04:10Acetone Fixation
  • 05:21Microscopic Imaging
  • 07:56Results: Visualization of C. elegans Exl-1 Subcellular Localization through GFP Tagging and DAPI Staining
  • 09:07Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议演示了如何在热应激下跟踪蛋白质核易位, 方法是使用绿色荧光蛋白 (GFP) 融合蛋白作为标记, 4 ', 6-diamidino-2-苯基吲哚 (DAPI) 染色。DAPI 染色协议快速, 保留了 GFP 和蛋白质亚细胞定位信号。

Tags

Protein Subcellular LocalizationGFP TaggingDAPI StainingCaenorhabditis ElegansCell BiologyTranslational ConstructExtrachromosomal ArrayGamma RadiationTransgenic LineStress AssayHeat StressM9 BufferSynchronizationL4 Larvae

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