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Journal / Biochemistry / Structure Solution of the Fluorescent Protein…
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Biochemistry

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JoVE Journal Biochemistry
Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect
 

Structure Solution of the Fluorescent Protein Cerulean Using MeshAndCollect

Article DOI: 10.3791/58594-v • 06:42 min • March 19th, 2019
March 19th, 2019 •
*1, *1, 2, 1, 1, 1, 1,3, 1, 1
1European Synchrotron Radiation Facility, Structural Biology Group, 2European Molecular Biology Laboratory, 3Univ. Grenoble Alpes, CNRS, CEA, IBS (Institut de Biologie Structurale)
* These authors contributed equally

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We present the use of the MeshAndCollect protocol to obtain a complete diffraction data set, for use in subsequent structure determination, composed of partial diffraction data sets collected from many small crystals of the fluorescent protein Cerulean.

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Keywords: MeshAndCollect Serial Crystallography Rotation Data Collection Small Crystals Puck Samples Structure Solution Ligand Screening Synchrotron Beamline VMX Protein Crystallography Beamline Sample Changer Data Collection Visual Reorientation Workflow
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