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Engineering

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Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
 

Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy

Article DOI: 10.3791/58781-v 08:39 min January 7th, 2019
January 7th, 2019

Capitoli

Riepilogo

Low-Intensity Pulsed Ultrasound Stimulation (LIPUS) is a modality for non-invasive mechanical stimulation of endogenous or engineered cells with high spatial and temporal resolution. This article describes how to implement LIPUS to an epi-fluorescence microscope and how to minimize acoustic impedance mismatch along the ultrasound path to prevent unwanted mechanical artefacts.

Tags

Keywords: Multiplexing Focused Ultrasound Stimulation Fluorescence Microscopy Mechanobiology Mechanical Forces Noninvasive Cell Culture Extracellular Matrix Polyester Film UV Sterilization Translation Stages Immersion Transducer
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