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Journal / Immunology and Infection / Isolation of Single Intracellular Bacterial…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis
 

Isolation of Single Intracellular Bacterial Communities Generated from a Murine Model of Urinary Tract Infection for Downstream Single-cell Analysis

Article DOI: 10.3791/58829-v • 07:34 min • April 16th, 2019
April 16th, 2019 •
1, 1, 2, 3, 3, 1,3
1Infectious Diseases Group, Genome Institute of Singapore, 2Department of Chemical and Biomolecular Engineering, National University of Singapore, 3Division of Infectious Diseases, Department of Medicine, Yong Loo Lin School of Medicine, National University of Singapore

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Automatic Translation

This protocol describes a simple method of isolating single, infected-bladder epithelial cells from a murine model of urinary tract infection.

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Keywords: Intracellular Bacterial Communities IBCs Urinary Tract Infection UTI Mouse Bladder Mouth Micropipetting Glass Capillary Tube Bladder Epithelial Cells Single-cell Analysis
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