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Immunology and Infection

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JoVE Journal Immunology and Infection
Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions
 

Kinetic Visualization of Single-Cell Interspecies Bacterial Interactions

Article DOI: 10.3791/61376-v • 08:33 min • August 5th, 2020
August 5th, 2020 •
*1, *1, 1
1Department of Microbiology and Immunology, Carver College of Medicine, University of Iowa
* These authors contributed equally

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This live-bacterial cell imaging protocol allows for visualization of interactions between multiple bacterial species at the single-cell level over time. Time-lapse imaging allows for observation of each bacterial species in monoculture or coculture to interrogate interspecies interactions in multispecies bacterial communities, including individual cell motility and viability.

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Keywords: Single-cell Interspecies Bacterial Interactions Kinetic Visualization Agarose Pads Cell Tracking Gene Expression Viability Staining Bacterial Growth Media Environmental Chamber Humidity Wipes Co-culture Microscopy
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