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Developmental Biology

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JoVE Journal Developmental Biology
A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats
 

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats

Article DOI: 10.3791/59260-v • 05:34 min • May 20th, 2019
May 20th, 2019 •
*1, *3, 2, 1, 1, 1,4, 2, 1
1Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 2Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, 3Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, 4Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University
* These authors contributed equally

Capitoli

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Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

Tags

Mini-invasive Internal Fixation Technique Knee Flexion Contracture Rats Muscle-gap Separation Model Reproducible Minimally Invasive Knee Joint Contraction Model Anesthetized Rat Disinfect Povidone Iodine Surgical Drape Femur Greater Trochanter Skin Incision Tissue Forceps Bluntly Dissect Retractor
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