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Developmental Biology

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JoVE Journal Developmental Biology
A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation
 

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Article DOI: 10.3791/56114-v • 08:46 min • July 26th, 2017
July 26th, 2017 •
1,2, 2,3, 2
1Department of Life Sciences, Peking University, 2National Institute of Biological Sciences, Beijing, 3Graduate School of Peking Union Medical College

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Internal lung surface area (ISA) is a critical criterion for assessing lung morphology and physiology in lung diseases and injury-induced alveolar regeneration. We describe here a standardized method that can minimize the measurement bias for ISA in both lung pneumonectomy and prosthesis implantation mouse models.

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Keyword Extraction: Mouse Pneumonectomy Prosthesis Implantation Internal Lung Surface Area Lung Function Mechanical Force-induced Adult Lung Regeneration Tracheal Intubation Thoracotomy Left Lung Lobe Removal Pulmonary Artery And Bronchi Ligation Chest Wall Closure Post-operative Care
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