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Developmental Biology

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JoVE Journal Developmental Biology
Whole-mount Confocal Microscopy for Adult Ear Skin
 

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

Article DOI: 10.3791/57406-v • 08:42 min • March 29th, 2018
March 29th, 2018 •
1,2, 1, 1
1Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, 2Earle A. Chiles Research Institute, Robert W. Franz Cancer Center, Providence Portland Medical Center

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Here, we describe a high resolution whole-mount imaging method in the entire adult mouse ear skin, which enables us to visualize branching morphogenesis and patterning of peripheral nerves and blood vessels, as well as immune cell distribution.

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Whole-mount Confocal Microscopy Adult Ear Skin Neuro-vascular Branching Morphogenesis Immune Cell Distribution Visualize Morphological Abnormalities Neurobiology Vascular Biology Immunology Peripheral Nerves Blood Vessels Inflammation Three-dimensional Architecture Juvenile To Adult Mice Normal And Pathology Conditions Euthanizing Adult Mice Dissect The Ear Petri Dish HBSS Trim The Hairs Posterior And Anterior Skin Intervening Cartilage 24-well Plate Ice-cold 4%PFA In PBS
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