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Measuring Enzymatic Stability by Isothermal Titration Calorimetry
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Measuring Enzymatic Stability by Isothermal Titration Calorimetry

Measuring Enzymatic Stability by Isothermal Titration Calorimetry

DOI:
10.3791/59302-v

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08:37 min

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March 26, 2019

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, , ,
1Department of Nutrition, Dietetics and Food Science,Brigham Young University, 2Department of Chemistry and Biochemistry,Brigham Young University

Capitoli

  • 00:04Titolo
  • 00:51Preparing Samples
  • 03:00Preparing the ITC (Isothermal Titration Calorimetry) Instrument
  • 05:22Setting up the ITCrun and Analyzing Data
  • 06:36Results: Enzymatic Stability
  • 08:04Conclusion

Summary

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The thermal stability of enzyme activity is readily measured by isothermal titration calorimetry (ITC). Most protein stability assays currently used measure protein unfolding, but do not provide information about enzymatic activity. ITC enables direct determination of the effect of enzyme modifications on the stability of enzyme activity.

Tags

Enzyme StabilityIsothermal Titration CalorimetryEnzymatic ReactionsEnergy-efficient CatalystsEnzyme ModificationsEnzyme AssaysSodium Acetate BufferEnzyme SolutionSubstrate Solution

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