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Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
JoVE Journal
Genetica
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JoVE Journal Genetica
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
DOI:

08:10 min

May 02, 2019

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Capitoli

  • 00:04Titolo
  • 01:37RNA Extraction and Reverse Transcription of the Viral RNA
  • 03:08First-round PCR
  • 04:03Second-round PCR
  • 04:41Analysis of the PCR Products by Electrophoresis on Agarose Gels
  • 05:48Results: Evaluation of the Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
  • 07:44Conclusion

Summary

Traduzione automatica

A pan-lyssavirus nested reverse transcription polymerase chain reaction has been developed to detect specifically all known lyssaviruses. Validation using rabies brain samples of different animal species showed that this method has a sensitivity and specificity equivalent to the gold standard fluorescent antibody test and could be used for routine rabies diagnosis.

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