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一种通用嵌套逆转录酶链反应检测裂解病毒的评价
JoVE Journal
Genetica
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JoVE Journal Genetica
Evaluation of a Universal Nested Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
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一种通用嵌套逆转录酶链反应检测裂解病毒的评价

DOI:
10.3791/59428-v

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08:10 min

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May 02, 2019

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, , , , , , ,
1Key Laboratory of Jilin Province for Zoonosis Prevention and Control, Institute of Military Veterinary Medicine,Academy of Military Medical Sciences, 2Jilin Agricultural University, 3Jiangsu Co-innovation Centre for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Capitoli

  • 00:04Titolo
  • 01:37RNA Extraction and Reverse Transcription of the Viral RNA
  • 03:08First-round PCR
  • 04:03Second-round PCR
  • 04:41Analysis of the PCR Products by Electrophoresis on Agarose Gels
  • 05:48Results: Evaluation of the Reverse Transcription Polymerase Chain Reaction for the Detection of Lyssaviruses
  • 07:44Conclusion

Summary

Traduzione automatica

Traduzione automatica

一种泛裂解病毒嵌套逆转录聚合酶链反应已开发出来, 专门检测所有已知的溶酶病毒。利用不同动物物种的狂犬病脑样本进行验证, 表明该方法具有与金标准荧光抗体检测相当的敏感性和特异性, 可用于常规狂犬病诊断。

Tags

LyssavirusNested RT-PCRUniversal DetectionAnimal Brain SpecimensBiological Fluid SpecimensRNA ExtractionReverse TranscriptionPCR ContaminationPositive ControlNegative ControlThermocycler

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