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Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments
 

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Article DOI: 10.3791/59514-v • 06:08 min • May 26th, 2019
May 26th, 2019 •
1, 2, 2, 1,2
1Department of Cancer Biology, Cleveland Clinic, 2Department of Radiation Oncology, Cleveland Clinic

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Clinical linear accelerators can be used to determine biologic effects of a wide range of dose rates on cancer cells. We discuss how to set up a linear accelerator for cell-based assays and assays for cancer stem-like cells grown as tumorspheres in suspension and cell lines grown as adherent cultures.

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Keywords: Linear Accelerator Radiation Therapy Radiobiology In Vitro Experiments Glioma Stem-like Cells Cell Culture Cell Irradiation Dosimetry Dosimetric Calculations Monitor Units
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