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通过流动细胞测定检测和描述 Vivo 中的蛋白质自组装

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Detecting and Characterizing Protein Self-Assembly In Vivo by Flow Cytometry

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通过流动细胞测定检测和描述 Vivo 中的蛋白质自组装

DOI:

10.3791/59577-v

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05:58 min

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July 17, 2019

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Shriram Venkatesan*¹, Tejbir S. Kandola*¹, Alejandro Rodríguez-Gama, Andrew Box, Randal Halfmann²

¹Stowers Institute for Medical Research, ²Department of Molecular and Integrative Physiology,The University of Kansas School of Medicine

Capitoli

00:04Titolo
00:56Saccharomyces cerevisiae Preparation
02:04Photoconversion and Distributed Amphifluoric Fluorescence Resonance Energy Transfer (DAmFRET) Data Collection
02:51Data Analysis
03:32Results: Representative In Vivo Protein Self-Assembly Detection and Characterization
05:26Conclusion

Summary

Traduzione automatica

本文介绍了一种基于FRET的流式细胞测定方案,用于量化S.cerevisae和HEK293T细胞中的蛋白质自组装。

Tags

Protein Self-assembly Flow Cytometry DAmFRET FRET Acceptor Signal Yeast Culture Induction Medium Photo Conversion Imaging Flow Cytometer Fluorescence Positive Gate

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