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Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells
 

Photostimulation by Femtosecond Laser Activates Extracellular-signal-regulated Kinase (ERK) Signaling or Mitochondrial Events in Target Cells

Article DOI: 10.3791/59661-v • 11:00 min • July 6th, 2019
July 6th, 2019 •
1,2, 2, 1, 3
1Department of Respiratory Medicine, Shanghai Sixth People's Hospital, School of Medicine, Shanghai Jiao Tong University, 2Ultrafast Laser Laboratory, College of Precision Instrument and Optoelectronics Engineering, Tianjin University, 3School of Biomedical Engineering, Shanghai Jiao Tong University

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Tightly-focused femtosecond laser can deliver precise stimulation to cells by being coupled into a confocal microscopy enabling the real-time observation and photostimulation. The photostimulation can activate cell molecular events including ERK signaling pathway and mitochondrial flashes of reactive oxygen species.

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Photostimulation Femtosecond Laser Extracellular-signal-regulated Kinase (ERK) Signaling Mitochondrial Events Optogenetics Cellular Molecular Signals Oral Optical Manipulation Confocal Microscope Single-cell Operation Subcellular-level Operation Real-time Molecular Dynamic Monitoring Fluorescent Microscopy Systems Laser Facility Lab Femtosecond Laser Source Reflective Mirrors Mechanical Shutter Relay Telescope Objective Focus
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