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Immunology and Infection

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JoVE Journal Immunology and Infection
Purification of a High Molecular Mass Protein in Streptococcus mutans
 

Purification of a High Molecular Mass Protein in Streptococcus mutans

Article DOI: 10.3791/59804-v • 09:51 min • September 14th, 2019
September 14th, 2019 •
1, 1, 1,2, 2, 1
1Department of Translational Research, Tsurumi University School of Dental Medicine, 2Laboratory for Oral Health Science

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Described here is a simple method for the purification of a gene product in Streptococcus mutans. This technique may be advantageous in the purification of proteins, especially membrane proteins and high molecular mass proteins, and can be used with various other bacterial species.

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Keywords: Protein Purification Streptococcus Mutans High Molecular Mass Protein PCR Genomic DNA Extraction Gel Electrophoresis Gel Extraction Nested PCR Electroporation Competent Cells Brain-heart Infusion Broth
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