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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E
 

Isolation of Lamina Propria Mononuclear Cells from Murine Colon Using Collagenase E

Article DOI: 10.3791/59821-v • 09:48 min • September 26th, 2019
September 26th, 2019 •
1,2,3,4, 1,2,3,4, 1,2,3,4, 1,2,3,4,5
1Department of Pediatrics, Division of Hematology / Oncology and Bone Marrow Transplantation, University of Miami Miller School of Medicine, 2Batchelor Children's Research Institute, University of Miami Miller School of Medicine, 3Department of Microbiology & Immunology, University of Miami Miller School of Medicine, 4Sylvester Comprehensive Cancer Center, University of Miami Miller School of Medicine, 5Holtz Children's Hospital, University of Miami Miller School of Medicine

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The goal of this protocol is to isolate mononuclear cells that reside in the lamina propria of the colon by enzymatic digestion of the tissue using collagenase. This protocol allows for the efficient isolation of mononuclear cells resulting in a single cell suspension which in turn can be used for robust immunophenotyping.

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Isolation Lamina Propria Mononuclear Cells Murine Colon Collagenase E Phenotypic Analysis Immune Cells Intestine GI And Systemic Inflammatory Disorders Viable Mononuclear Cells Contaminating Debris Immune Phenotyping Fleur's Atomistry Mouse Models Cancer Allergy Transplantation Autoimmunity Yield Efficiency Solutions Materials Euthanizing The Mouse Vertical Midline Incision Peritoneum Small Bowel Descending Colon Rectal Portion Of The Colon Distal Rectum Cecal Cap Moistened Paper Towel Scissors Or Forceps Mild Pressure Solid Stool
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