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JoVE Journal Genetics
Dérivation et traitement de la tumorale à partir de cellules tumorales primaires isolées de la souris Rhabdomyosarcomas
 
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Dérivation et traitement de la tumorale à partir de cellules tumorales primaires isolées de la souris Rhabdomyosarcomas

Article DOI: 10.3791/59897-v • 09:21 min • September 13th, 2019
September 13th, 2019 •
1,2,3, 2
1Graduate School of Biomedical Sciences, Sanford Burnham Prebys Medical Discovery Institute, 2Development, Aging and Regeneration Program, Sanford Burnham Prebys Medical Discovery Institute, 3Department of Obstetrics, Gynecology, and Reproductive Sciences, Division of Female Pelvic Medicine and Reconstructive Surgery, University California San Diego

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Ce protocole décrit une méthode reproductible pour l'isolement des cellules primaires de rhabdomyosarcoma de souris, de la formation et du traitement de la tumorsphere, et de la transplantation d'allogreffe commençant des cultures de tumorspheres.

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Génétique Numéro 151 tumorspheres rhabdomyosarcoma muscle squelettique cellules primaires traitement des protéines recombinantes transfection plasmide
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