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评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C）损伤的反应

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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo

Assessment of Oxidative Damage in the Primary Mouse Ocular Surface Cells/Stem Cells in Response to Ultraviolet-C (UV-C) Damage

Please note that all translations are automatically generated. Click here for the English version.

评估原小鼠眼部表面细胞/干细胞对紫外线-C （UV-C）损伤的反应

DOI:

10.3791/59924-v

•

12:59 min

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February 15, 2020

•

Bipasha Bose*¹, Saketh Kapoor*¹, Utsav Sen*¹, Muhammad Nihad AS, Debajit Chaudhury, Sudheer Shenoy P

¹Stem Cells and Regenerative Medicine Centre,Yenepoya Research Centre, Yenepoya (Deemed to be University), Mangaluru-575018, Karnataka, India

Capitoli

00:00Introduction
01:36Ultraviolet C (UV-C) Radiation Exposure to Mouse Primary Ocular Cells
03:43Preparation of the Living-Cell Staining Media
04:58Live Cell Staining of UV-C Exposed Mouse Primary Ocular Cells
05:51Viewing of DCFDA (ROS), Hoechst and PI Stained Cells
07:40Risultati
11:57Conclusion

Summary

Traduzione automatica

该协议演示了从小鼠眼部表面细胞同时检测活原培养物中的活性氧物种（ROS）、活细胞和死细胞。2'，7'-二氯氟乙酸酸酯，碘化钠和Hoechst染色分别用于评估ROS，死细胞和活细胞，然后成像和分析。

Tags

Keywords: Oxidative Damage Primary Mouse Ocular Surface Cells Stem Cells Ultraviolet-C (UV-C) Damage Reactive Oxygen Species (ROS)DCFDA Propidium Iodide Hoechst Live Cell Permeant Dead Cell Permeant UV Crosslinker Dose-response

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