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Journal / Developmental Biology / Serum Free Production of Three-dimensional Human…
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Developmental Biology

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JoVE Journal Developmental Biology
Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells
 

Serum Free Production of Three-dimensional Human Hepatospheres from Pluripotent Stem Cells

Article DOI: 10.3791/59965-v • 06:57 min • July 20th, 2019
July 20th, 2019 •
1, 1,2, 1,3, 1,4, 1, 1, 4, 3, 1
1MRC Centre for Regenerative Medicine, University of Edinburgh, 2UCL Great Ormond Street Institute of Child Health, University College London, 3Division of Biosciences, Department of Life Sciences, College of Health and Life Sciences, Brunel University London, 4School of Biochemistry and Immunology, Trinity Biomedical Sciences Institute, Trinity College Dublin

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Automatic Translation

This protocol describes an approach to produce hepatospheres from human pluripotent stem cells using a defined culture system and cell self-assembly. This protocol is reproducible in a number of cell lines, cost effective and allows the production of stable human hepatospheres for biomedical application.

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Keywords: 3D Hepatospheres Pluripotent Stem Cells Serum-free Agarose Molds Cell Suspension Rock Inhibitor Cell Culture Tissue Engineering
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