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JoVE Journal Neuroscience
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
 

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

Article DOI: 10.3791/59982-v • 08:13 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1,2, 1
1Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 2Department of Cellular Neurophysiology, Institute of Physiology CAS

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This article presents a method to study glutamate receptor (GluR) trafficking in dissociated primary hippocampal cultures. Using an antibody-feeding approach to label endogenous or overexpressed receptors in combination with pharmacological approaches, this method allows for the identification of molecular mechanisms regulating GluR surface expression by modulating internalization or recycling processes.

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Keywords: Antibody Feeding Glutamate Receptor Trafficking Primary Hippocampal Cultures Surface Expression Internalization Recycling Paraformaldehyde Fixation Fab Anti-IgG Dynasore
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