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Immunology and Infection

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JoVE Journal Immunology and Infection
Adoptivimmunterapi af iNKT-celler i glucose-6-fosfatisomerase (G6PI)-Induceret RA-mus
 
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Adoptivimmunterapi af iNKT-celler i glucose-6-fosfatisomerase (G6PI)-Induceret RA-mus

Article DOI: 10.3791/60048-v • 08:43 min • January 31st, 2020
January 31st, 2020 •
*1,2, *1,2, *1,2, *1,2, *1,2, *1,2, *1,2, *1,2, *1,2
1Medical School of Hebei University, 2Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases in Hebei Province
* These authors contributed equally

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Denne protokol bruger G6PI blandede peptider til at konstruere leddegigt modeller, der er tættere på den menneskelige leddegigt i CD4+ T celler og cytokiner. Høj renhed invariant naturlige dræber T-celler (hovedsagelig iNKT2) med specifikke fænotyper og funktioner blev opnået ved in vivo induktion og in vitro rensning for adoptivimmunterapi.

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Immunologi og infektion leddegigt adoptivimmunterapi iNKT-celler cytokiner glucose-6-fosfat isomerase (G6PI) in vivo imaging system (IVIS)
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