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Immunology and Infection

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JoVE Journal Immunology and Infection
A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model
 

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Article DOI: 10.3791/60229-v • 11:03 min • October 30th, 2019
October 30th, 2019 •
1, 1, 1, 1, 1, 1
1Immunology, Janssen Research & Development, Inc.

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A cryogenic pulverization method to process murine paws using a liquid nitrogen freezer mill was developed to improve the yield and quality of RNA or protein extracted from the tissues and enable the analysis of molecular profiles associated with inflammatory responses.

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Keywords: Cryo-pulverization Tissue Inflammation Collagen-induced Arthritis Model Molecular Profiling Proteomic Analysis Transcriptional Profiling Tissue Homogenization Tissue Dissociation RNA Isolation Complex Tissue Environment Arthritis Research Preclinical Model Tissue Processing Freezer Mill Liquid Nitrogen
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