Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Detection of Human Immunodeficiency Virus Type 1…
JoVE Journal
Genetics

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Genetics
Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR
 

Detection of Human Immunodeficiency Virus Type 1 (HIV-1) Antisense Protein (ASP) RNA Transcripts in Patients by Strand-Specific RT-PCR

Article DOI: 10.3791/60511-v • 08:01 min • November 27th, 2019
November 27th, 2019 •
1, 1, 2, 3, 4, 5, 2, 1, 1
1Division of Immunology and Allergy, Lausanne University Hospital, 2Department of Fundamental Neuroscience, University of Lausanne, 3Department of Biochemistry, Erasmus Medical Center, 4Theoretical Biology and Biophysics Group, Los Alamos National Laboratories, 5Department of Biochemistry, University of Lausanne

Capitoli

Riepilogo
Automatic Translation

RNA hairpins and loops can function as primers for reverse transcription (RT) in absence of sequence-specific primers, interfering with the study of overlapping antisense transcripts. We have developed a technique able to identify strand-specific RNA, and we have used it to study HIV-1 antisense protein ASP.

Tags

Keywords: HIV-1 Antisense Protein (ASP) RNA Transcripts Strand-specific RT-PCR Sense And Antisense Sequences Biotinylated Primers RNA Denaturation Anti-sense CDNAs Overlapping Genes Quantification DNase Treatment Reverse Transcription Endogenous RT Controls Reaction Mixture Magnetic Beads Washing And Binding Buffer
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter