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The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells
 

The CryoAPEX Method for Electron Microscopy Analysis of Membrane Protein Localization Within Ultrastructurally-Preserved Cells

Article DOI: 10.3791/60677-v • 11:45 min • February 27th, 2020
February 27th, 2020 •
1, 2, 2,3,4, 1,4,5,6
1Department of Biological Sciences, Purdue University, 2Department of Medicinal Chemistry and Molecular Pharmacology, Purdue University, 3Purdue Institute of Inflammation, Immunology and Infectious Disease, Purdue University, 4Bindley Biosciences Center, Purdue University, 5Purdue Institute of Integrative Neuroscience, Purdue University, 6Purdue Center for Cancer Research, Purdue University

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This protocol describes the cryoAPEX method, in which an APEX2-tagged membrane protein can be localized by transmission electron microscopy within optimally-preserved cell ultrastructure.

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CryoAPEX Method Electron Microscopy Analysis Membrane Protein Localization Ultrastructurally-Preserved Cells Staining Membrane Integrity Subcellular Architecture Genetically Cloneable Tag APEX2 Cellular Preservation Cryofixation Freeze Substitution Protein Localization Viruses Replicate Host Proteins Label Proteins Golgi Breakdown CryoAPEX Technique Immunofluorescence Techniques
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