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Immunology and Infection

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JoVE Journal Immunology and Infection
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
 

Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System

Article DOI: 10.3791/60693-v • 09:12 min • March 10th, 2020
March 10th, 2020 •
1, 1,2, 3, 1,2, 1
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine, Yale University School of Medicine, 2Microbial Sciences Institute, Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School, The University of Texas Health Science Center at Houston

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Imaging of bacterial cells is an emerging systems biology approach focused on defining static and dynamic processes that dictate the function of large macromolecular machines. Here, integration of quantitative live cell imaging and cryo-electron tomography is used to study Legionella pneumophila type IV secretion system architecture and functions.

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Live Cell Imaging Cryo-electron Tomography Legionella Pneumophila Dot/Icm Secretion System Bacterial Secretion Complexes Host-pathogen Interactions Native Structure Protein Complexes Agarose Pads Low Melt Agarose Solution Glass Slides Air Bubbles
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