Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

Nan Chen; Kai Zhu; Shiqiang Yan; Junmin Li; Tianyi Pan; Mieradilijiang Abudupataer; Fazle Alam; Xiaoning Sun; Li Wang; Chunsheng Wang

doi:10.3791/60920

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Bioingegneria

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Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

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Bioingegneria

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JoVE Journal Bioingegneria

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

DOI:

10.3791/60920-v

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06:29 min

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May 02, 2020

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Nan Chen², Kai Zhu², Shiqiang Yan⁴, Junmin Li², Tianyi Pan, Mieradilijiang Abudupataer², Fazle Alam, Xiaoning Sun², Li Wang⁴, Chunsheng Wang²

¹Department of Cardiac Surgery and Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital,Fudan University, ²Shanghai Institute of Cardiovascular Diseases, ³Center for Medical Research and Innovation, Shanghai Pudong Hospital,Fudan University Pudong Medical Center, ⁴Institutes of Biomedical Sciences and Department of Systems Biology for Medicine, Shanghai Medical College,Fudan University

Capitoli

00:04Introduction
01:05Cell-Laden Silk Fibroin-Gelatin Methacryloyl (SF-GelMA) Preparation
03:28SF-MultiLayered-GelMA Loading, Reheating and Bioprinting
05:16Results: Representative Bioprinting Results Using Three Different Cell-Laden Bioinks
05:59Conclusion

Summary

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Here, we developed a novel multilayered modified strategy for liquid-like bioinks (gelatin methacryloyl with low viscosity) to prevent the sedimentation of encapsulated cells.

Using Multilayered Hydrogel Bioink in Three-Dimensional Bioprinting for Homogeneous Cell Distribution

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