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Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber
 

Longitudinal In Vivo Imaging and Quantification of Human Pancreatic Islet Grafting and Contributing Host Cells in the Anterior Eye Chamber

Article DOI: 10.3791/61234-v • 09:07 min • June 11th, 2020
June 11th, 2020 •
1,2, 1,2, 1,2
1Department of Experimental Medical Science, Lund University, 2Lund University Diabetes Centre

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The goal of this protocol is to continuously monitor the dynamics of the human pancreatic islet engraftment process and the contributing host versus donor cells. This is accomplished by transplanting human islets into the anterior chamber of the eye (ACE) of an NOD.(Cg)-Gt(ROSA)26Sortm4-Rag2-/-mouse recipient followed by repeated 2-photon imaging.

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Keywords: In Vivo Imaging Pancreatic Islet Grafting Anterior Eye Chamber Beta Cell Replacement Therapy Islet Transplantation Two-photon Microscopy Islet Function And Viability Diabetic Complications Real-time Imaging
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