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Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
 

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage

Article DOI: 10.3791/61236-v • 06:51 min • May 6th, 2020
May 6th, 2020 •
1, 1, 2
1Department of Biology, Emmanuel College, 2Department of Chemistry and Physics, Emmanuel College

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A highly parallel method for measuring the site-specific cleavage of DNA at the single molecule level is described. This protocol demonstrates the technique using the restriction endonuclease NdeI. The method can easily be modified to study any process that results in site-specific DNA cleavage.

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Keywords: DNA Cleavage Single Molecule Kinetics High Throughput DNA Binding DNA Modification DNA Target Search Flow Cell Tethering PCR Buffer A Anti-digoxigenin Fab Fragments
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