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JoVE Journal Cancer Research
An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity
 

An Automated Differential Nuclear Staining Assay for Accurate Determination of Mitocan Cytotoxicity

Article DOI: 10.3791/61295-v • 07:58 min • May 12th, 2020
May 12th, 2020 •
*1, *1, 1
1Department of BioSciences, Rice University
* These authors contributed equally

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The protocol describes a rapid, high-throughput, reliable, inexpensive, and unbiased assay for efficiently determining cellular viability. This assay is particularly useful when cells' mitochondria have been damaged, which interferes with other assays. The assay uses automated counting of cells stained with two nuclear dyes – Hoechst 33342 and propidium iodide.

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Automated Differential Nuclear Staining Assay Mitocan Cytotoxicity Cellular Viability Mitochondrial Function High Throughput Drug Screening Fast Accurate Inexpensive Compounds Cytotoxicity Drug Preparation Treatment Conditions Cell Density Experimental Accuracy Validity Visual Demonstration Image Acquisition Image Analysis Gen5 Software Compound Concentration Solvent Controls Trypan Blue Staining
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