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JoVE Journal Neuroscience
Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles
 

Culture of Neurospheres Derived from the Neurogenic Niches in Adult Prairie Voles

Article DOI: 10.3791/61402-v • 07:34 min • June 10th, 2020
June 10th, 2020 •
1, 2, 1, 1,3, 4, 1
1Departamento de Neurobiología Conductual y Cognitiva, Instituto de Neurobiología, Universidad Nacional Autónoma de México, 2Silvio O. Conte Center for Oxytocin and Social Cognition, Center for Translational Social Neuroscience, Department of Psychiatry and Behavioral Sciences, Yerkes National Primate Research Center, Emory University, 3Escuela Nacional de Estudios Superiores Juriquilla, Universidad Nacional Autónoma de México, 4Departamento de Fisiología y Desarrollo Celular, Instituto Nacional de Perinatología

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We established the conditions to culture neural progenitor cells from the subventricular zone and dentate gyrus of the adult brain of prairie voles, as a complementary in vitro study, to analyze the sex-dependent differences between neurogenic niches that could be part of functional plastic changes associated with social behaviors.

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Keywords: Neurospheres Neurogenic Niches Prairie Voles Neural Stem Cells Progenitor Cells Subventricular Zone Dentate Gyrus Microdissection Enzymatic Dissociation Cell Culture Proliferation Differentiation
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