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Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish
 

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Article DOI: 10.3791/61575-v • 13:25 min • May 15th, 2021
May 15th, 2021 •
*1, *1, *1,2, 1, 1, 1, 1,2
1Department of Immunology, University of São Paulo, 2Department of Medicine, Nephrology Division, Federal University of São Paulo
* These authors contributed equally

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Automatic Translation

This protocol describes the procedures to induce Acute Kidney Injury (AKI) in adult zebrafish using cisplatin as a nephrotoxic agent. We detailed the steps to evaluate the reproducibility of the technique and two techniques to analyze inflammation and cell death in the renal tissue, flow cytometry and TUNEL, respectively.

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Keywords: Acute Kidney Injury Cisplatin Zebrafish Nephrotoxicity Inflammation Cell Death Flow Cytometry TUNEL Assay Chemotherapy Renal Protection Regeneration
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