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Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy
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JoVE Journal Medicina
Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

DOI:
10.3791/61857-v

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09:52 min

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September 21, 2021

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, , , ,
1Oregon Hearing Research Center, Department of Otolaryngology/Head & Neck Surgery,Oregon Health & Science University

Capitoli

  • 00:04Introduction
  • 02:11Preparation of the Fluorescent-Labeled Blood Cells and Surgery to Create an Open Window
  • 05:39Imaging of Cochlear Blood Flow Under a Fluorescence Intravital Microscope and Video Analysis
  • 07:54Results: IVM Imaging of Cochlear Capillaries and Analysis of Change in Vessel Diameter and Blood Flow in the Stria
  • 09:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

An open vessel-window approach using fluorescent tracers provides sufficient resolution for cochlear blood flow (CoBF) measurement. The method facilitates the study of structural and functional changes in CoBF in mouse under normal and pathological conditions.

Tags

Cochlear Blood FlowMeniere’s DiseaseHearing LossFluorescence MicroscopyTransgenic Mouse ModelCardiac PunctureBlood Cell LabelingSurgical ProcedureAnesthesiaVital Signs Monitoring

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