Journal
/
Medicine
/
Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy
JoVE Journal
Medicine
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Medicine
Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

Measurement of Strial Blood Flow in Mouse Cochlea Utilizing an Open Vessel-Window and Intravital Fluorescence Microscopy

DOI:
10.3791/61857-v

•

09:52 min

•

September 21, 2021

•

, , , ,
1Oregon Hearing Research Center, Department of Otolaryngology/Head & Neck Surgery,Oregon Health & Science University

Chapters

  • 00:04Introduction
  • 02:11Preparation of the Fluorescent-Labeled Blood Cells and Surgery to Create an Open Window
  • 05:39Imaging of Cochlear Blood Flow Under a Fluorescence Intravital Microscope and Video Analysis
  • 07:54Results: IVM Imaging of Cochlear Capillaries and Analysis of Change in Vessel Diameter and Blood Flow in the Stria
  • 09:21Conclusion

Summary

Automatic Translation

Automatic Translation

An open vessel-window approach using fluorescent tracers provides sufficient resolution for cochlear blood flow (CoBF) measurement. The method facilitates the study of structural and functional changes in CoBF in mouse under normal and pathological conditions.

Tags

Cochlear Blood FlowMeniere’s DiseaseHearing LossFluorescence MicroscopyTransgenic Mouse ModelCardiac PunctureBlood Cell LabelingSurgical ProcedureAnesthesiaVital Signs Monitoring

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/61857?article_type=v

Visualization and Analysis of Blood Flow and Oxygen Consumption in Hepatic Microcirculation: Application to an Acute Hepatitis Model
check_url/61857?article_type=v

An Orthotopic Glioblastoma Mouse Model Maintaining Brain Parenchymal Physical Constraints and Suitable for Intravital Two-photon Microscopy
check_url/61857?article_type=v

Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice
check_url/61857?article_type=v

Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats
check_url/61857?article_type=v

Evaluation of Planar-Cell-Polarity Phenotypes in Ciliopathy Mouse Mutant Cochlea
check_url/61857?article_type=v

Combined Intravital Microscopy and Contrast-enhanced Ultrasonography of the Mouse Hindlimb to Study Insulin-induced Vasodilation and Muscle Perfusion
check_url/61857?article_type=v

Intravital Microscopy and Thrombus Induction in the Earlobe of a Hairless Mouse
check_url/61857?article_type=v

A Chronic Cardiac Ischemia Model in Swine Using an Ameroid Constrictor
check_url/61857?article_type=v

Simultaneous Electrocardiography Recording and Invasive Blood Pressure Measurement in Rats
check_url/61857?article_type=v

Simultaneous Flow Cytometric Characterization of Multiple Cell Types Retrieved from Mouse Brain/Spinal Cord Through Different Homogenization Methods

Read Article