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Journal / Bioengineering / Multi-timescale Microscopy Methods for the…
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Bioengineering

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JoVE Journal Bioengineering
Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release
 

Multi-timescale Microscopy Methods for the Characterization of Fluorescently-labeled Microbubbles for Ultrasound-Triggered Drug Release

Article DOI: 10.3791/62251-v • 06:02 min • June 12th, 2021
June 12th, 2021 •
1, 1,2, 1, 3, 4,5,6, 3,6,7, 7, 1
1Physics of Fluids group, Department of Science and Technology, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 2BIOS Lab-on-a-Chip group, Max Planck Center Twente for Complex Fluid Dynamics, MESA+ Institute for Nanotechnology and Technical Medical (TechMed) Center, University of Twente, 3Department of Biotechnology and Nanomedicine, SINTEF Industry, 4Department of Circulation and Medical Imaging, Norwegian University of Science and Technology, 5Department of Health Research, SINTEF Digital, 6Cancer Clinic, St. Olav’s Hospital, 7Department of Physics, Norwegian University of Science and Technology

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The presented protocols can be used to characterize the response of fluorescently-labeled microbubbles designed for ultrasound-triggered drug delivery applications, including their activation mechanisms as well as their bioeffects. This paper covers a range of in vitro and in vivo microscopy techniques performed to capture the relevant length and timescales.

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Keywords: Microbubbles Ultrasound Drug Delivery Fluorescence Microscopy Brightfield Microscopy Multi-timescale Characterization
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