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Immunology and Infection

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JoVE Journal Immunology and Infection
Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging
 

Longitudinal Follow-Up of Urinary Tract Infections and Their Treatment in Mice using Bioluminescence Imaging

Article DOI: 10.3791/62614-v • 07:39 min • June 14th, 2021
June 14th, 2021 •
1, 2, 1, 1, 1, 3, 1, 4
1Laboratory of Ion Channel Research (LICR), VIB-KU Leuven Center for Brain & Disease Research, Leuven, Belgium & Department of Cellular and Molecular Medicine, KU Leuven, 2Biomedical MRI, Department of Imaging & Pathology, KU Leuven, 3Belgium & KU Leuven Centre of Microbial and Plant Genetics, VIB-KU Leuven Center for Microbiology, Leuven, 4Laboratory of Experimental Urology, Department of Development and Regeneration, KU Leuven

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This manuscript describes the intravesical administration of uropathogenic bacteria with a lux operon to induce a urinary tract infection in mice and subsequent longitudinal in vivo analysis of the bacterial load using bioluminescence imaging.

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Keywords: Bioluminescence Imaging Urinary Tract Infections UTI Longitudinal Follow-up Bacterial Culture Inoculum Preparation Anesthesia Intravesical Instillation
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