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Biochemistry

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JoVE Journal Biochemistry
Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor
 

Extraction and Visualization of Protein Aggregates after Treatment of Escherichia coli with a Proteotoxic Stressor

Article DOI: 10.3791/62628-v • 07:59 min • June 29th, 2021
June 29th, 2021 •
1, 1, 1, 1
1School of Biological Sciences, Illinois State University

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This protocol describes the extraction and visualization of aggregated and soluble proteins from Escherichia coli after treatment with a proteotoxic antimicrobial. Following this procedure allows a qualitative comparison of protein aggregate formation in vivo in different bacterial strains and/or between treatments.

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Keywords: Protein Aggregation Proteotoxic Stress Escherichia Coli Protein Extraction Visualization Cell Lysis Glass Bead Disruption MOPS-g Medium OD600 Antimicrobial Compound
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