Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Bioengineering / Automated Microbial Cultivation and Adaptive…
JoVE Journal
Bioengineering

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Bioengineering
Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)
 

Automated Microbial Cultivation and Adaptive Evolution using Microbial Microdroplet Culture System (MMC)

Article DOI: 10.3791/62800-v • 08:18 min • February 18th, 2022
February 18th, 2022 •
*1,2, *1,2,3, 4, 1,2,6, 1,2,5, 3, 1,2,5
1Department of Chemical Engineering, Institute of Biochemical Engineering, Tsinghua University, 2Key Laboratory of Industrial Biocatalysis, Ministry of Education, Tsinghua University, 3Luoyang TMAXTREE Biotechnology Co., Ltd., 4Biochemical Engineering Research Group, School of Chemical Engineering and Technology, Xi’an Jiaotong University, 5Center for Synthetic & Systems Biology, Tsinghua University, 6School of Life Science and Technology, Tokyo Institute of Technology
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

This protocol describes how to use the Microbial Microdroplet Culture system (MMC) to conduct automated microbial cultivation and adaptive evolution. MMC can cultivate and sub-cultivate microorganisms automatically and continuously and monitor online their growth with relatively high throughput and good parallelization, reducing labor and reagent consumption.

Tags

Automated Microbial Cultivation Adaptive Evolution Microbial Microdroplet Culture System Low-cost Operation-friendly Result-reliable Experimental Platform Labor Consumption Reduction Culture Performance Microbiology Research Fields Growth Curve Measurement Adaptive Laboratory Evolution Single Factor Multi-level Analysis Syringe Needle Quick Connector A Reagent Bottle Autoclave Microfluidic Chip Operation Chamber Optical Fiber Probe Positioning Columns
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter