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Cancer Research

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JoVE Journal Cancer Research
Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research
 

Mammary Epithelial and Endothelial Cell Spheroids as a Potential Functional In vitro Model for Breast Cancer Research

Article DOI: 10.3791/62940-v • 08:30 min • July 12th, 2021
July 12th, 2021 •
1, 1, 2, 3, 1, 1, 1,3
1Department of Reproductive Endocrinology, Wagistrasse 14, University of Zurich and University Hospital Zurich, 2Department of Dermatology, Wagistrasse 18, University of Zurich and University Hospital Zurich, 3Department of Pharmacology and Chemical Biology, University of Pittsburgh

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Crosstalk between mammary epithelial cells and endothelial cells importantly contributes to breast cancer progression, tumor growth, and metastasis. In this study, spheroids have been made from breast cancer cells together with vascular and/or lymphatic endothelial cells and demonstrate their applicability as an in vitro system for breast cancer research.

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Keywords: Breast Cancer Cell Spheroids 3D Model Mammary Epithelial Cells Endothelial Cells Cancer Cell-endothelial Cell Interactions Proliferation Metastasis In Vitro Model Cell Distribution Dye Staining Enzymatic Detachment Cell Counting Cell Suspension Cell Co-culture
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