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Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model
 

Multiphoton Intravital Imaging for Monitoring Leukocyte Recruitment during Arteriogenesis in a Murine Hindlimb Model

Article DOI: 10.3791/62969-v • 07:50 min • September 30th, 2021
September 30th, 2021 •
1,2,3, 4, 1,5, 1,3, 1,3, 1,5
1Walter-Brendel-Centre of Experimental Medicine, University Hospital, Ludwig-Maximilians-Universität München, 2Department of Otorhinolaryngology, Head & Neck Surgery, University Hospital, Ludwig-Maximilians-Universität München, 3Biomedical Center, Institute of Cardiovascular Physiology and Pathophysiology, Faculty of Medicine, Ludwig-Maximilians-Universität München, 4TKI, University of Bern, 5Department of Internal Medicine I and Cardiology, University Hospital, Ludwig-Maximilians-Universität München

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The recruitment of leukocytes and platelets constitutes an essential component necessary for the effective growth of collateral arteries during arteriogenesis. Multiphoton microscopy is an efficient tool for tracking cell dynamics with high spatio-temporal resolution in vivo and less photo-toxicity to study leukocyte recruitment and extravasation during arteriogenesis.

Tags

Multiphoton Intravital Imaging Leukocyte Recruitment Arteriogenesis Murine Hindlimb Model Immune Cell Adherence Extravasation Multiphoton Microscopy Technique Cell Dynamics Deep Tissue Living Mouse Models Phototoxicity Spatiotemporal Resolution Microscope Procedure Surgical Procedure Molded Clay Supine Position Stereo Microscope Femoral Artery Ligation Scar Skin Removal Fat Layer Removal Adductor Muscle Profunda Artery Profunda Vein Collateral Vessels
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