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Immunology and Infection
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JoVE Journal Immunology and Infection
Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays
 

Use of Crystal Violet to Improve Visual Cytopathic Effect-based Reading for Viral Titration using TCID50 Assays

Article DOI: 10.3791/63063-v • 04:06 min • February 12th, 2022
February 12th, 2022 •
1, 1
1College of Veterinary Medicine, Department of Population Health and Pathobiology, North Carolina State University

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This protocol shows an accurate and objective approach to visualize viral titrations using crystal violet, by comparing it with optical microscopy and immunocytochemical staining.

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Keywords: Viral Titration TCID50 Assay Crystal Violet Staining Cytopathic Effect Virus Stock Cell Line Serial Dilution Viral Titer Microscopy Monocytochemistry Staining Plaque-forming Unit Assay Virology Research
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