Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Developmental Biology / Microdissection and Dissociation of the Murine…
JoVE Journal
Developmental Biology

È necessario avere un abbonamento a JoVE per visualizzare questo Contenuto. Accedi o inizia la tua prova gratuita.

JoVE Journal Developmental Biology
Microdissection and Dissociation of the Murine Oviduct
 

Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

Article DOI: 10.3791/63168-v • 07:44 min • November 4th, 2021
November 4th, 2021 •
1, 1, 2, 1
1Division of Biomedical Sciences, School of Medicine, University of California, Riverside, 2Microscopy and Imaging Core Facility, University of California, Riverside

Capitoli

Riepilogo
Automatic Translation

A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.

Tags

Keywords: Microdissection Dissociation Murine Oviduct Segment Identification Single Cell Isolation Ampulla Infundibulum Stromal Cells Epithelial Cells Immune Cells Smooth Muscle Cells In Vitro Fertilization Malignant Transformation Tissue Dissection Tissue Dissociation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter