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Immunology and Infection

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JoVE Journal Immunology and Infection
Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System
 

Culturing Lymphocytes in Simulated Microgravity Using a Rotary Cell Culture System

Article DOI: 10.3791/63296-v • 09:28 min • August 25th, 2022
August 25th, 2022 •
1,2, 1,2,4, 1,3
1Laboratory Medicine and Pathobiology, Temerty Faculty of Medicine, University of Toronto, 2Krembil Research Institute, Toronto Western Hospital, University Health Network, 3Pathology and Lab Medicine, Hematopathology, Mount Sinai Hospital, Sinai Health Systems, 4Medical Oncology and Hematology, Princess Margaret Cancer Centre

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This is a step-by-step guide for using a commercially available rotary cell culture system to culture lymphocytes in simulated microgravity using specialized disposable culture vessels. This culturing method may be applied to any suspension-type cell culture.

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Keywords: Lymphocytes Simulated Microgravity Rotary Cell Culture System Immune Cells Space Environment Human Physiology Molecular Level Economically Feasible Cell Culture Control Treatment Complete Media Incubator Flask Control T25 Suspension Culture Flask Stock Cell Culture Syringe Port Caps Stopcocks Fill Port Serological Pipette Vacuum System
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