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Biochemistry

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JoVE Journal Biochemistry
Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry
 

Analyzing the Interaction of Fluorescent-Labeled Proteins with Artificial Phospholipid Microvesicles using Quantitative Flow Cytometry

Article DOI: 10.3791/63459-v • 08:26 min • April 6th, 2022
April 6th, 2022 •
1,2, 1,4, 1,2,3, 1,2, 1,2,3,4
1Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics, Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology

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Here, we describe a set of methods for characterizing the interaction of proteins with membranes of cells or microvesicles.

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Fluorescent-labeled Proteins Artificial Phospholipid Microvesicles Quantitative Flow Cytometry Kinetic Interaction Equilibrium Interaction Protein Binding PPH Membrane Reagents And Equipment Availability Basic Research Protein-lipid Interactions Blood Coagulation Ligand Binding Dynamics Kinetic Binding Experiments Tyrode's Buffer Fluorescence Measurements
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