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Analyse de l’interaction des protéines marquées fluorescence avec des microvésicules de phospholipides artificielles à l’aide de la cytométrie quantitative en flux…
JoVE Journal
Biochemistry
Biochemistry
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Analyse de l’interaction des protéines marquées par fluorescence avec des microvésicules de phospholipides artificielles à l’aide de la cytométrie quantitative en flux
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Article
DOI:
10.3791/63459-v
•
08:26 min
•
April 6th, 2022
Analyse de l’interaction des protéines marquées par fluorescence avec des microvésicules de phospholipides artificielles à l’aide de la cytométrie quantitative en flux
April 6th, 2022
•
Nadezhda Podoplelova1,2, Polina Soloveva1,4, Andrei Garzon Dasgupta1,2,3, Aleksandra Filkova1,2, Mikhail Panteleev1,2,3,4
1Center for Theoretical Problems of Physicochemical Pharmacology, Russian Academy of Sciences, 2National Medical Research Center of Pediatric Hematology, Oncology and Immunology named after Dmitry Rogachev, 3Faculty of Physics, Lomonosov Moscow State University, 4Faculty of Biological and Medical Physics, Moscow Institute of Physics and Technology
Capitoli
Riepilogo
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Ici, nous décrivons un ensemble de méthodes pour caractériser l’interaction des protéines avec les membranes de cellules ou de microvésicules.
