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Bioengineering

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JoVE Journal Bioengineering
Formulering og karakterisering af et eksosombaseret dopaminbærersystem
 
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Formulering og karakterisering af et eksosombaseret dopaminbærersystem

Article DOI: 10.3791/63624-v • 06:08 min • April 4th, 2022
April 4th, 2022 •
1,2, 3,4,5,6, 2,7, 2, 2, 8, 8, 3,4,5,6,9
1Vocational School of Health Services/İstanbul, Altinbas University, 2Chemical Metallurgy Faculty, Department of Bioengineering, Yildiz Technical University, 3Faculty of Medicine, Department of Histology & Embryology, Istinye University, 43D Bioprinting Design & Prototyping R&D Center, Istinye University, 5Stem Cell and Tissue Engineering R&D Center, Istinye University, 6Medicine Faculty, Istinye University, 7Vocational School of Health Services, Istinye University, 8The V. Akhundov National Scientific Research Medical Prophylactic Institute, 9Center for Stem Cells and Regenerative Medicine (LivMedCell), Liv Hospital

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Her sigtede vi mod at opnå en formulering ved dopaminindlæsning af de isolerede exosomer af stamceller fra Whartons gelé mesenkymale stamceller. Exosomisolering og karakterisering, lægemiddelbelastning i de resulterende exosomer og den cytotoksiske aktivitet af den udviklede formulering er beskrevet i denne protokol.

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Exosom-baseret dopaminbærersystem ekstracellulære vesikler intercellulær last kommunikation kropsvæsker blod-hjerne-barriere nanobærersystem indkapsling af dopamin Whartons gelémesenkymale stamceller lægemiddelfrigivelse cytotoksicitetsassays fibroblastlevedygtighed
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