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Developmental Biology

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JoVE Journal Developmental Biology
Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing
 

Detection and Quantitation of Label-Retaining Cells in Mouse Incisors using a 3D Reconstruction Approach after Tissue Clearing

Article DOI: 10.3791/63721-v • 10:32 min • June 10th, 2022
June 10th, 2022 •
*1, *1, 1, 1
1Department of Biomedical Sciences, Texas A&M School of Dentistry
* These authors contributed equally

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The mouse incisor contains valuable label-retaining cells in its stem cell niche. We have a novel way to unbiasedly detect and quantify the label-retaining cells; our study used EdU labeling and a 3D reconstruction approach after PEGASOS tissue clearing of the mandible.

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Keywords: 3D Reconstruction Label-retaining Cells Mouse Incisor Stem Cell Niche Tissue Clearing 5-ethynyl-2'-deoxyuridine (EdU) Staining Whole Mount Decalcification Decolorization De-lipidation Tert-butanol-polyethylene Glycol (tB-PEG) Benzyl Benzoate-polyethylene Glycol (BB-PEG) Clearing Confocal Imaging
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