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Developmental Biology

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JoVE Journal Developmental Biology
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
 

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Article DOI: 10.3791/63834-v • 07:26 min • April 1st, 2022
April 1st, 2022 •
*1, *1, 1
1Department of Craniofacial Biology, School of Dental Medicine, University of Colorado Anschutz Medical Campus
* These authors contributed equally

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The protocol presents a method for isolating whole cell protein lysates from dissected mouse embryo facial processes or cultured mouse embryonic palatal mesenchyme cells and performing subsequent western blotting to assess phosphorylated protein levels.

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Keywords: Protein Lysates Phosphoprotein Analysis Facial Processes Palatal Mesenchyme Cells Craniofacial Development Mouse Embryos Protein Isolation Phosphatase Inhibitors Protein Phosphorylation Dissection Tissue Processing Cell Culture
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